S8320

Image

1 of 1


S8320

Sigma

 

SigmaMarker™

High Range, mol wt 36,000-200,000 Da

MDL number:MFCD00165844

Details

Related Products

References

Reviews

Description

ApplicationSigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.
Other NotesProtein (mol. wt.)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
β-Galactosidase, E. coli (116,000)
Myosin, porcine heart (200,000)
 Will yield bands for the following seven proteins from the SigmaMarker list with Coomassie Blue staining: glyceraldehyde-3-phosphate dehydrogenase, ovalbumin, glutamic dehydrogenase, albumin, phosphorylase b, β-galactosidase and myosin.
ReconstitutionReconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 0.5% dithiothreitol, 2% SDS, and 0.0005% bromophenol blue.
Legal InformationSigmaMarker is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

formlyophilized powder
usage sufficient for 10-20 large gels (16 × 18 cm)
  sufficient for 20-30 mini-gels (10 × 10 cm)
  vial sufficient for 50-100 PhastGels
mol wtmol wt 36,000-200,000 Da
storage temp.2-8°C

Safety

WGK Germany3

References

referenceLaemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970) Abstract
 Schagger, H. and von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368-379, (1987)